Translational_Unit

Part:BBa_K1932001:Design

Designed by: Jin Liu   Group: iGEM16_Jilin_China   (2016-10-14)


orf1 and orf2 from Bifidobacterium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 935


Design Notes

(1)The restriction enzyme cutting site were identified. (2)The sequences of ori and orf were confirmed.


Source

the plasmid of PMB1 from Bifidobacterium

References